ck2 inhibitor, cx-4945 (silmitasertib  (ApexBio)

Journal: Current biology : CB

Article Title: A Ca 2+ -Dependent Switch Activates Axonal Casein Kinase 2α Translation and Drives G3BP1 Granule Disassembly for Axon Regeneration

doi: 10.1016/j.cub.2020.09.043

Figure Lengend Snippet: (A) Exposure-matched epifluorescent images for CK2α, G3BP1, and G3BP1PS149 in DRGs transfected with Cnsk2a1/2 (siCsnk2a1/2) and control (siCon) siRNAs.

Article Snippet: CK2 inhibitor (CX-4945) , Selleckchem , S2248.

Techniques: Transfection, Control

Journal: Current biology : CB

Article Title: A Ca 2+ -Dependent Switch Activates Axonal Casein Kinase 2α Translation and Drives G3BP1 Granule Disassembly for Axon Regeneration

doi: 10.1016/j.cub.2020.09.043

Figure Lengend Snippet: (A and B) Exposure-matched confocal images for CK2α in naive and 24 h post-injured sciatic nerve from wild-type versus mTOR 3′ UTR−/− mice shown in (A). Upper rows of image pairs show CK2α merged with NF signals in single XY planes; lower rows show XYZ projections of CK2α signals that overlap with NF (axonal CK2α) across individual XY planes. (B) shows quantitation of axonal CK2α from sciatic nerve axons as mean ± SEM (N = 3 animals; **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 between the indicated groups by one-way ANOVA with Tukey HSD post hoc; scale bar represents 10 μm).

Article Snippet: CK2 inhibitor (CX-4945) , Selleckchem , S2248.

Techniques: Quantitation Assay

Journal: Current biology : CB

Article Title: A Ca 2+ -Dependent Switch Activates Axonal Casein Kinase 2α Translation and Drives G3BP1 Granule Disassembly for Axon Regeneration

doi: 10.1016/j.cub.2020.09.043

Figure Lengend Snippet: (A) Schematic for experimental approach to test for CK2α effects on signaling to G3BP1 for axon growth.

Article Snippet: CK2 inhibitor (CX-4945) , Selleckchem , S2248.

Techniques:

Journal: Current biology : CB

Article Title: A Ca 2+ -Dependent Switch Activates Axonal Casein Kinase 2α Translation and Drives G3BP1 Granule Disassembly for Axon Regeneration

doi: 10.1016/j.cub.2020.09.043

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CK2 inhibitor (CX-4945) , Selleckchem , S2248.

Techniques: Virus, Isolation, Recombinant, Conjugation Assay, Software

Journal: eLife

Article Title: Limited inhibition of multiple nodes in a driver network blocks metastasis

doi: 10.7554/eLife.59696

Figure Lengend Snippet:

Article Snippet: For in vitro and in vivo studies, JNK inhibitor SP600125, MEK inhibitor Trametinib (GSK1120212), MLK inhibitor URMC-099, and CK2 inhibitor CX-4945 (Silmitasertib) were purchased from APExBIO (A4604, A3018, B4877, A833010, respectively). p38 inhibitor SB203580 was purchased from Selleckchem (Cat No S1076) for the in vitro experiments.

Techniques: Derivative Assay, Blocking Assay, Software, Western Blot, Transfection, Construct, Sequencing, Recombinant, Plasmid Preparation, Control, Marker, Chemotaxis Assay, Invasion Assay, Membrane, Concentration Assay